Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: The blue arrow pointing at the vessel in diet induced obesity sparague upper right portion of the image on the left side is enlarged and shown on the right side.
Limitations[ edit ] Different limitations of the diet-induced obesity model. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats.
The mice who drank Goishi tea gained less weight and had less sugar in their blood than the mice who drank tap water and green tea. Author information: However, a direct comparison between Wistar and SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far.
Feeding rats a high fat diet for up to 32 weeks did not affect endoneurial blood flow Figure 2 B. In studies with A. For the study of these pathologies, a number of animal models have been developed. Additionally, obesity remains in both species for long periods of time after it has initially set in.
For instance, casein causes more weight gaining compared to soy. A qualitative assessment of sciatic nerves by light microscopy did not identify any overt pathological damage to axons or any changes in myelin structure such as splitting, ballooning or thinning.
Then, the number of intraepidermal nerve fibre profiles was determined as described in the Methods section.
Previously, in type 1 and type 2 diabetic rats, we found that impaired vasodilation to acetylcholine occurred early and preceded slowing of endoneurial blood flow and MNCV 450 To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study.
The minimum number of rats in each group is 9 We had previously demonstrated that epineurial arterioles are innervated by sensory nerves that contain CGRP and that CGRP is a potent vasodilator in epineurial arterioles To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study.
Given the growing body of evidence demonstrating the prominent role of gut microbiota in energy balance and metabolism, the gut microbiota composition and its modulation by HF diet were also evaluated in both strains.
Research done with model[ edit ] A summary of how a study on diet induced obesity in mice was performed. Mice[ edit ] An obese mouse and a normal weight mouse Mice are used by scientists as diet-induced obesity models in experiments because they have mammalian physiological systems similar to those in humans.
Figure 3 Open in figure viewer PowerPoint Effect of a high fat diet on intraepidermal nerve fibre profiles Rats were fed a standard or high fat diet for 32 weeks.
However, the majority of these effects were more pronounced or earlier detected in Wistar rats. However, the majority of these effects were more pronounced or earlier detected in Wistar rats.
However, there is precedence in models of toxic neuropathies, such as pyridoxine intoxication, in which sensory nerves are more prone to dysfunction For these studies, control rats were age matched for each time point. In studies by my laboratory and others, reduced endoneurial blood flow has been shown to precede slowing of MNCV 17182750 - Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat.
Data in Figure 8 demonstrate that expression of NEP was increased about 2. The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR.
The number of intraepidermal nerve fibre profiles in a skin biopsy from the right hindpaw was significantly decreased in rats fed a high fat diet for 32 weeks compared with that in control rats Figure 3 bottom.As in most science experiments, a good experiment using the diet-induced obesity model has an experimental and a control group.
The control group is given a diet with low percentage of total energy from fat (e.g. 10%), while the experimental group is given a diet with a high percentage of total energy from fat (e.g.
Environment-Induced Obesity. In addition to genetic mutation-induced obese animal models, diet-induced obesity (DIO) is also widely accepted to study the mechanisms that contribute to obesity-induced cardiac injury and dysfunction (Sciarretta et al., ; Xu et al., ). The Effect of Diet-induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model.
The consumption of a high energy (HE) diet causes a range of weight gain responses in the human population with some individuals resistant to diet induced obesity (DIO).Author: Z Archer, D Rayner, J Mercer.
RESEARCH PAPER High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat Claudia Marques a, b, Manuela Meireles a,Sonia Norberto, Joana Leite, Joana Freitas,c, Diogo Pestana. · High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat.
Marques C(1), Meireles M(2), Norberto S(2), Leite J(2), Freitas J(3), Pestana D(1), Faria A(4), Calhau C(1).Cited by: